Figure 8.

FLC Alters the Peak Level of LUX in a Temperature-Specific Manner.

The temporal pattern in LUX transcript abundance was analyzed by real-time PCR in FRI; FLC (closed diamonds) and fri; flc (open diamonds) genotype seedlings at 27°C (A) and at 22°C (B). LUX expression levels were normalized with respect to ACT2, and the average for the fri; flc genotype at each temperature across ZT32 to ZT40 was set to 1. Data shown are the average of two to three biological replicates, with technical triplicates (see Methods). Error bars represent se.