Figure 2.

Functional Test of orf79 in Male Sterility.

(A) The binary construct for expression of recombinant orf79 in rice. P_35S, the CaMV35S promoter; Rf1b-5′, the 5′ segment of Rf1b encoding mitochondrion transit signal.

(B) to (D) Pollen grains of a normal fertile rice line (B) and orf79 transgenic T0 rice plants with single (C) or two (D) T-DNA insertions. The darkly stained pollens were fertile and the lightly stained were sterile. Bars = 50 μm.

(E) Cosegregation of the orf79 transgene and the semi-male-sterility (s) in a T1 family with single T-DNA insertion as assayed by RNA gel blot analysis of young panicle RNA using orf79 as a probe. f, full male fertility.