Figure 6.
ConcA Induces Intracellular BRI1-GFP Accumulation.
Cells in the root elongation zone expressing BRI1-GFP were stained with the endocytic tracer FM4-64. Shown are the GFP channels ([A], [C], [E], and [G]) and overlays of the separately recorded GFP (green) and FM4-64 (red) channels ([B], [D], [F], and [H]). Bars = 10 μm.
(A) and (B) Untreated cells expressing BRI1-GFP stained with FM4-64 for 10 min.
(C) and (D) Cells expressing BRI1-GFP treated with ConcA for 2 h in the presence of FM4-64.
(E) and (F) Cells expressing BRI1-GFP treated with CHX for 1 h in the presence of FM4-64.
(G) and (H) Cells expressing BRI1-GFP treated with CHX for 1 h followed by treatment with ConcA + CHX for 1 h in the presence of FM4-64.