Figure 3.

Elicitation of FLS2-Dependent Plant Responses by Purified His-Tagged Flagellins.

(A) Example of the recombinant His-tagged flagellin samples used in this study. On Coomassie blue–stained SDS-PAGE gels, lane 1 carried E. coli pQE30-fliC total lysate after isopropyl β-d-thiogalactoside induction, and lanes 2 to 5 show four independent samples of His-tagged flagellin after elution from nickel-nitrilotriacetic acid agarose columns.

(B) FLS2-dependent elicitation of Arabidopsis defense responses, assayed as seedling growth inhibition. Seedlings of Arabidopsis Col-0 (shaded bars) and fls2-101 mutant (open bars) were treated with water (mock) as a negative control, 10 μM flg22 peptide as a positive control, or 5 μM purified His-tagged flagellin of Xcc strain B186, B94, or B305. Means ± se are shown.

(C) Arabidopsis Col-0 seedlings were treated with water, 10 μM flg22, or 5 μM purified His-tagged flagellin of Xcc strain B186 or B305, S. enterica serovar Typhimurium strain LT2, or R. solanacearum strain K60. Means ± se are shown.