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. 2006 Mar;18(3):764–779. doi: 10.1105/tpc.105.037648

Figure 5.

Figure 5.

Identification and Dissection of Amino Acid Residue 43 as the Key Polymorphic Determinant of Xcc Flagellin for Arabidopsis Defense Elicitation.

(A) Defense elicitation activity of purified His-tagged flagellins carrying one, two, or three amino acid changes as noted, with defense elicitation assayed as seedling growth inhibition. Arabidopsis Col-0 seedlings were treated with water (mock), 10 μM flg22, or 5 μM of the designated purified His-tagged flagellin. Means ± se are shown.

(B) PR-1 gene expression activated by flg22 peptide or by purified His-tagged flagellins that carry the Asp-43 residue, consistent with the results shown in (A). Data are for semiquantitative PCR performed on Arabidopsis Col-0 or fls2-101 mutant seedling samples taken 24 h after exposure to elicitor. Actin expression was monitored as a control.

(C) Defense elicitation activity of purified His-tagged flagellins carrying different amino acids at Xcc flagellin residue 43, assayed as for (A). Means ± se are shown.