Figure 8.
Restriction of Pst and Xcc Growth as a Result of Leaf Elicitation with Purified His6-Tagged Flagellin Protein.
(A) Leaf populations of Pst DC3000 (means ± se) after 2 d of growth on leaves that were exposed to purified flagellin 24 h before inoculation with bacteria. Arabidopsis wild-type Col-0 (shaded bars) and fls2-101 (open bars) leaves were treated by syringe infiltration with water (mock) or with 1 μM of the His-tagged flagellins FliC305, FliC186, FliC186V43D, or FliC305D43V, then inoculated the next day with 105 cfu/mL Pst DC3000 by syringe infiltration.
(B) Leaf populations of Xcc after 2 d of growth on leaves that were exposed to purified flagellins 24 h before inoculation with 5 × 105 cfu/mL Xcc B186, as in (A).