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. 2006 Feb 28;103(10):3775–3780. doi: 10.1073/pnas.0600040103

Fig. 1.

Fig. 1.

Validation of the automated cell motility assay. (A) The temporal (0, 4, 8, 12, and 16 h) migration of SKOV-3 cells in the presence and absence of controls is shown. siCON, FITC-conjugated control siRNA; siRAC, a sequence-specific siRNA targeting RAC; DMSO, dimethyl sulfoxide; SRC, c-Src family kinase inhibitor, compound 43 (5). (B) Quantification of SKOV-3 migration. The degree of scratch closure (“migration score”) was determined by an automated algorithm and plotted as a function of time. Low migration scores reflect migratory inhibition. (C) Western blot demonstrating knockdown of the RAC protein by the RAC-specific siRNA used in A, compared to a control siRNA (CON) and mock transfected cells (LIPO). The same blot reprobed with an anti-actin antibody to demonstrate equal loading is shown below.