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Decrease in JNK phosphorylation after siRNA-mediated knockdown of MAP4K4. (A) Western blots of SKOV-3 total protein lysates collected 48 h after transfection with control (si_C) or MAP4K4-specific siRNAs (si_1 and si_2). Blots were probed with phospho-specific antibodies (p) and antibodies recognizing total JNK, p38, ERK, and STAT-3. (B) Scratch migration assay of SKOV-3 cells exposed to increasing concentrations of the JNK inhibitor, SP600125.
