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. 2005 Dec 12;393(Pt 1):59–67. doi: 10.1042/BJ20050674

Figure 7. Immunoelectron microscopy analysis of Cx43 localization in cells treated with bafilomycin A1 and PMA.

Figure 7

To identify lysosomes, cells were incubated with 8 nm BSA–gold for 3 h and chased overnight. IAR20 cells were treated with bafilomycin A1 (200 nM) for 30 min prior to and during treatment with PMA (100 ng/ml) for 60 min, fixed, prepared for electron microscopy and labelled for Cx43 (15 nm gold). (A) Cx43 often localized in electron-lucent vacuoles with few internal vesicles, which resembled early endosomes. Cx43 was, under these conditions, found both on the limiting membrane of the vacuole and on the internal vesicles. (B–D) Cx43 was also found in more electron-lucent vacuoles containing several internal vesicles. Sometimes these vacuoles contained what appeared to be remnants of gap junction double membranes (arrowheads). (E) Cx43 labelling in multivesicular endosome that appear to fuse with a lysosome. (F) Cx43 labelling in enlarged multivesicular endosome with 8 nm gold particles. Scale bars, 200 nm.