Skip to main content
. 2005 Dec 12;393(Pt 1):79–87. doi: 10.1042/BJ20050808

Figure 2. Detection of a rat Abca5 splice variant.

Figure 2

(A) Structural organization of the rat Abca5 gene on chromosome 10. Exons are numbered in ascending order from the 5′-terminus, starting with the putative first untranslated exon and are depicted as equally sized black boxes. Exon 20, which is extended by 16 bp in the rat Abca5 splice variant rAbca5 V20+16, is marked with an asterisk. A metric scale in corresponding kb is indicated. (B) Enlarged view of the cDNA region in human ABCA5 and rat Abca5 between exons 20 and 21. The conserved 16 bp insertions leading to a premature stop codon in the splice variant of both species are boxed. The premature stop codon and deviating bases are underlined. (C) Design of RTQ PCR assay for selective amplification of rAbca5 V20+16 (EMBL accession no. AJ550165) cDNA. The regions corresponding to the primers and Taqman probe RTQ1 are boxed. The PCR reaction amplifying total rAbca5 and splice variant rAbca5 V20+16 cDNA fragments used primers RTQ.forw and RTQ.rev. To amplify solely the rAbca5 V20+16 cDNA fragment, RTQ.forw and SVRTQ.rev were employed.