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. 2005 Dec 12;393(Pt 1):79–87. doi: 10.1042/BJ20050808

Figure 6. Expression of organelle marker proteins in transiently transfected HEK-293 cells (magnification, ×630).

Figure 6

Images show HEK-293 cells after transfection with 0.1–0.5 μg of marker expression plasmid per 60 mm culture dish. (A) Cells transfected with pECFP-Mem (0.1 μg), giving rise to a protein containing the N-terminal 20 amino acids of neuromodulin (GAP-43) fused to the N-terminus of ECFP (plasma membrane and intracellular distribution), 72 h after transfection. (B) Cells transfected with pDsRed-Mito (0.25 μg), yielding a protein containing the mitochondrial targeting sequence of cytochrome c oxidase subunit VIII, fused to DsRed, 48 h after transfection. (C) Cells transfected with pDsRed-Endo (0.5 μg), yielding a protein containing the ER-targeting sequence of calreticulin, fused to DsRed, 48 h after transfection. (D) Cells transfected with pDsRed2-Peroxi (0.25 μg), leading to a protein of DsRed2, fused to the peroxisomal targeting signal 1 (SKL), 72 h after transfection. (E, F) Cells transfected with pDsRed-Golgi (0.25 μg), expressing a protein exhibiting the Golgi-targeting sequence within the N-terminus of β-1,4-galatosyltransferase, fused to DsRed, 48 h (E) and 24 h (F) after transfection.