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. 2005 Dec 12;393(Pt 1):79–87. doi: 10.1042/BJ20050808

Figure 7. Co-localization of rAbca5 fusion proteins with Golgi marker (magnification, ×630).

Figure 7

HEK-293 cells were co-transfected with either transporter-EGFP expression plasmid (prAbca5–EGFP or prAbca5 V20+16–EGFP) and pDsRed-Golgi or, alternatively, with the plasmid prAbca5-V5 (expressing V5 epitope-tagged rAbca5) and pDsRed-Golgi, as outlined in the Experimental section. Expression of fusion proteins was documented separately after 24 h (full rAbca5–EGFP), 36 h (rAbca5 V20+16–EGFP) and 48 h (full rAbca5–V5) respectively and merged using Openlab software (Improvision).