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. 2005 Dec 12;393(Pt 1):141–150. doi: 10.1042/BJ20050327

Figure 1. Gel shift analysis of binding reactions using fragment BP as a probe and head (HE) or trunk (TE) embryo extracts from different ages (10.5, 11.5 and 13.5 dpc).

Figure 1

Specificity of DNA–protein complexes is demonstrated by competition reactions using 100 times unlabelled BP (lanes HE+C and TE+C) and by competition reactions using 100 times unlabelled mutant oligonucleotide DNA for the SMAD consensus site (lanes HE+M and TE+M). Each lane indicated as ‘P’ corresponds to migration of the probe alone. The arrows show the specific binding.