Skip to main content
. 2005 Dec 12;393(Pt 1):171–180. doi: 10.1042/BJ20051037

Figure 3. Extracellular nucleotides enhance cell migration of 3T3-L1 preadipocytes.

Figure 3

Cell migration assay was performed by the method described in Experimental section. The number of migrated cells is indicated as a percentage of basal migration. (A) Cells were incubated in the upper well with the complete culture medium (basal; Bas), with 1–100 μM ATP, 100 μM ADP, 100 μM UTP or 100 μM suramin (SRM) for 4 h or 100 μM suramin for 30 min followed by 3.5 h with 100 μM ATP in the continued presence of suramin (SRM+ATP). (B) Cells were incubated in the upper well with the complete culture medium (basal), with 100 μM ATP, 5 μM U-73122 or 5 μM U-73343 for 4 h, or 5 μM U-73122 for 30 min followed by 3.5 h with 100 μM ATP in the continued presence of U-73122 (U-73122+ATP), or 5 μM U-73343 for 30 min followed by 3.5 h with 100 μM ATP in the continued presence of U73343 (U-73343+ATP). Each value represents the mean±S.E.M. for three separate experiments. Statistical significance was determined by one-way ANOVA followed by Bonferroni's test.