Figure 4. Brief hypoxia enhances CO activity in NCMs.

Cells were exposed to hypoxia for the time periods shown followed by isolation of mitochondria (see the Experimental section). Mitochondria were then assayed for CO activity in the absence (hatched bars) or presence (open bars) of 1 mM D-β-M detergent. Results shown are means±S.E.M. for a single experiment conducted in duplicate and are representative of four identical experiments, each taken from a separate myocyte preparation.