Open arrows indicate ORFs and the directions of their transcripts. The probe synthesized by PCR using catB-N and catB-I primers was used for colony hybridization. The primers used for RT-PCR and primer extension analyses are indicated by horizontal arrows. The downward solid triangle indicates a transcriptional start site of cat gene cluster. A, ApaI; C, ClaI; E, EcoNI; N, NcoI; S, SacI.