The enzyme (24 units) and markers (2 mg of each) were placed on the top of a column (3.0 cm×60 cm) of Toyopearl HW-55S, and then eluted with buffer A containing 0.2 M NaCl. 1, Cytochrome c (12.5 kDa); 2, chymotrypsinogen A (25 kDa); 3, ovalbumin (45 kDa); 4, BSA (68 kDa); ●, CatB.