Figure 6. Agarose gel electrophoresis of RT-PCR product.

Total RNA prepared from succinate-grown cells of Rhodococcus sp. AN-22 was used for the synthesis of cDNA as a template. PCR was performed with the synthesized cDNA using OF and OR primers as amplification primers (lane 3). Control experiments were as follows: PCR was carried out using total RNA as a template without a reverse transcription step (lane 2) and using total DNA (lane 4) as a template. A 1-kb DNA ladder (New England Biolabs) was used as markers (lane 1). Sizes are given in kb.