Table 2. Purification of CatB from succinate-grown cells of Rhodococcus sp. AN-22.
Fraction | Total activity (units) | Total protein (mg) | Specific activity (units/mg) | Recovery (%) |
---|---|---|---|---|
Cell extract | 240 | 1800 | 0.14 | 100 |
Streptomycin sulphate | 200 | 2600 | 0.078 | 82 |
Ammonium sulphate | 220 | 470 | 0.48 | 92 |
DE52 | 140 | 51 | 2.7 | 57 |
DEAE-Toyopearl 650S | 140 | 6.4 | 22 | 58 |
HW-55S | 80 | 1.6 | 50 | 33 |
Phenyl-Toyopearl 650S | 42 | 0.81 | 52 | 18 |