Table 4. Effects of various compounds on the activity of CatB.
The enzyme (0.7 units) was incubated with various compounds in 1.0 ml of buffer A at 24 °C for 10 min. An aliquot (0.1 ml) of the incubation mixture was subjected to enzyme assay using cis,cis-muconate as a substrate. PCMB, p-chloromercuribenzoic acid.
| Compound | Concentration (mM) | Remaining activity (%) |
|---|---|---|
| None | – | 100 |
| HgCl2 | 1.0 | 20 |
| CuSO4·5H2O | 1.0 | 65 |
| Iodoacetic acid | 0.2 | 58 |
| PCMB | 0.1 | 92 |
| α,α′-Dipyridyl | 1.0 | 93 |
| N-Ethylmaleimide | 1.0 | 90 |
| Tiron | 1.0 | 100 |
| EDTA | 1.0 | 101 |
| o-Penanthroline | 0.05 | 101 |
| NaN3 | 1.0 | 96 |