Table 6. Purification of CatC from succinate-grown cells of Rhodococcus sp. AN-22.
Fraction | Total activity (units) | Total protein (mg) | Specific activity (units/mg) | Recovery (%) |
---|---|---|---|---|
Cell extract | 440 | 480 | 0.92 | 100 |
Streptomycin sulphate | 500 | 480 | 1.0 | 110 |
Ammonium sulphate | 320 | 71 | 4.5 | 73 |
DE52 | 100 | 7.2 | 14 | 23 |
DEAE-Toyopearl 650S | 75 | 1.8 | 42 | 17 |
Phenyl-Toyopearl 650S | 12 | 0.014 | 860 | 2.7 |