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. 2005 Dec 12;393(Pt 1):235–243. doi: 10.1042/BJ20051022

Figure 5. Reduced 3′-phosphoinositide turnover rate in G12V Ras-expressing cells.

Figure 5

Individual NIH 3T3 cells were co-transfected with GFP–AktPH and either G12V Ras or control vector and stimulated with 10 nM PDGF for 10 min, followed by inhibition of PI3K with wortmannin, and the ensuing decay in 3′-phosphoinositide level was monitored in real time using TIRF microscopy [25,30]. A total of seven control and 23 G12V Ras cells, from 4 days of experiments, were analysed. (A) Normalized TIRF time courses, following PI3K inhibition, for the control cells. (B) Whisker plot of estimated first-order 3′-phosphoinositide turnover rate constants for the two cell populations, depicting the median, upper and lower quartiles, and range of values for each population. Turnover was significantly slower in G12V Ras cells (P<0.001, Student's t test).