Table 1. Oligonucleotide primers used in PCR amplification to construct different Stau mutants and/or fusion proteins.

The restriction enzyme sequences are italicized in the sequences.

Name	Template	Oligonucleotide sequences	Restriction sites
pNIA-Stau1	RSV-Stau1	5′-AATTGGTACCTGCACTGTGCGTGAAACTTGGA	KpnI
		5′-TATAGTCGACGGCCAGAAAAGGT	SalI
PNIAE2-Nterm	RSV-Stau1	5′-ACCAATGTATAAGCCTGTTGACCCTTAC	−
		5′-TCAAGAACAGCTATGGCATTTT	−
pNIAE2-NLSs	RSV-Stau1	5′-CGCCATAGCTGTTCTTGA	−
		5′-TTTCCAACCTTCACCTG	−
pNIAE2-NLSbipartite	RSV-Stau1	5′-CGCCATAGCTGTTCTTGA	−
		5′-GGGCTTGTCTGTGGCTTGACTATGGGTT	−
pNIAE2-NLSbasic	RSV-Stau1	5′-GCCAGAGTACACGCTCCTCACAGAG	−
		5′-TTTCCAACCTTCACCTG	−
pNIAE2-Cterm	RSV-Stau1	5′-CACTGCAGAAGGAACGGGCACCAAC	−
		5′-CACCTCCCACACACAGACATTGGTCC	−
RBD2–YFP	RSV-Stau1	5′-TAGATATCGAATTCGCCGCCATGGGATACCCATTTCCAG	EcoRI
		5′-TATACTCGAGGATATCCAGGATCCTCAA	EcoRV
RBD3–YFP	RSV-Stau1	5′-TAGATATCGAATTCGCCGCCATGGGAAAATCTGAAATAAGT	EcoRI
		5′-TATACTCGAGGATATCAACTGCAGGCAGG	EcoRV
RBD3*F–YFP	RSV-St/3*F	5′-TAGATATCGAATTCGCCGCCATGGGAAAATCTGAAATAAGT	EcoRI
		5′-TATACTCGAGGATATCAACTGCAGGCAGG	EcoRV
RBD4–YFP	RSV-Stau1	5′-TAGATATCGAATTCGCCGCCATGGGAAATCCGATTAGC	EcoRI
		5′-TATACTCGAGGATATCAAGGATCTCCAG	EcoRV
RBD3*F/RBD4–YFP	RSV-St/3*F	5′-TAGATATCGAATTCGCCGCCATGGGAAAATCTGAAATAAGT	EcoRI
		5′-TATACTCGAGGATATCAAGGATCTCCAG	EcoRV
RBD2/RBD3	RSV-Stau1	5′-TAGATATCGAATTCGCCGCCATGGGATACCCATTTCCAG	EcoRI
		5′-TATACTCGAGGATATCAACTGCAGGCAGG	EcoRV
St/3*F/ΔN–YFP	St/3*F–YFP	5′-TAGATATCGAATTCGCCGCCATGGGAAAATCTGAAATAAGT	EcoRV
		5′-TATACTCGAGGATATCGCACCTCCCACACAC
St/3*F/Δ2–YFP	Stau1–YFP	5′-TATAGGTACCGCCACCATGGCGTTGAGGATC	KpnI
		5′-TATACTCGAGGATATCGCACCTCCCACACAC	−