Table 1. Binding of Gleheda to human blood group A, B, H, P1 Lea and Leb active gps, sialo and asialo gps as determined by ELLSA.
Biotinylated Gleheda (10 ng) was added to various gp concentrations ranging from 0.01 ng to 1 μg/ml.
| Panel in Figure 1 | Gp (lectin determinants*; blood group specificity) | Mass for 1.5 units of A405 (ng) | Maximum A405 reading† | Binding intensity† |
|---|---|---|---|---|
| Exposed multivalent Tn gps | ||||
| a | Asialo HSM (Tn) | 0.07 | 3.8 | +++++ |
| a | Native ASG-Tn (Tn) | 0.2 | 3.6 | +++++ |
| b | Asialo PSM (Tn, Tα, Ah, H) | 0.2 | 3.3 | +++++ |
| b | Asialo OSM (Tn) | 0.3 | 3.8 | +++++ |
| b | Asialo BSM (Tn, GlcNAcβ1-3Tn) | 0.4 | 3.4 | +++++ |
| a | Tn-Glycophorin (Tn) | 3.6 | 3.9 | +++++ |
| Cryptic multivalent Tn gps | ||||
| a | ASG-A (sialyl Tn) | 0.6 | 3.5 | +++++ |
| b | OSM (sialyl Tn) | 2.0 | 2.0 | ++++ |
| b | BSM (sialyl Tn, sialyl GlcNAcβ1-3Tn) | 10.0 | 1.8 | +++ |
| b | PSM (sialyl Tn, Tα, Ah, H) | 20.0 | 1.8 | +++ |
| a | HSM (sialyl Tn, Tn) | 130.0 | 1.6 | +++ |
| Blood group precursor equivalent gps (mixtures of exposed Tn, T, I, II) | ||||
| c | Cyst Mcdon P-1 | 2.5 | 3.1 | +++++ |
| c | Cyst Beach P-1 | 5.8 | 2.7 | +++++ |
| c | Cyst Tighe P-1 | 6.0 | 1.9 | +++ |
| c | Cyst MSS first Smith degraded | 22.0 | 2.0 | ++++ |
| Blood group ABH-active gps | ||||
| d | Sheep hydatid cyst gp (E[P1], Fα, crypto Fα and Tn) | 180.0 | 2.1 | ++++ |
| d | Cyst MSS 10% 2× (Ah [A1]) | − | 1.4 | ++ |
| d | Cyst 19 (B) | − | 1.3 | ++ |
| c | Cyst Mcdon (Ah) | − | 1.2 | ++ |
| c | Cyst Beach phenol-insoluble (B) | − | 1.2 | ++ |
| d | Cyst 9 phenol-insoluble (Ah) | − | 0.5 | + |
| d | Cyst JS phenol-insoluble (H) | − | 0.1 | − |
| c | Cyst Tighe phenol-insoluble (H, Leb) | − | 0.0 | − |
| T, crypto T, multi-antennary II-containing gps and polysaccharide | ||||
| e | Human glycophorin (sialyl Tα) | − | 0.1 | − |
| e | Asialo human glycophorin (Tα) | − | 0.9 | + |
| e | Inactive antifreeze gp [Tα; molecular mass (2.6–3.8)×103] | − | 0.2 | ± |
| e | Active antifreeze gp [Tα; molecular mass (1.0–2.1)×104] | − | 0.2 | ± |
| f | Pneumococcus type 14 polysaccharide (II) | − | 0.2 | ± |
| f | Human α1-acid gp (sialyl mII) | − | 0.1 | − |
| f | Asialo human α1-acid gp (mII) | − | 0.2 | ± |
| f | Fetuin (sialyl II, T) | − | 0.3 | ± |
| f | Asialo fetuin (II, T) | − | 0.0 | − |
| e | Mannan | − | 0.1 | − |
* The symbol in parentheses indicates the human blood group activity and/or lectin determinants [43]. m, multivalent.
† Results were interpreted according to the measured A405 after 2 h of incubation as follows: +++++, A405≥2.5; ++++, 2.5>A405≥2.0; +++, 2.0>A405≥1.5; ++, 1.5>A405≥1.0; +, 1.0>A405≥0.5; ±, 0.5>A405≥0.2 and −, A405<0.2.