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. 2005 Dec 12;393(Pt 1):331–341. doi: 10.1042/BJ20051162

Table 4. Gleheda binding to the Consortium glycan (glycotope) array.

Spacer definitions and entire glycan array version at http://www.functionalglycomics.org/static/consortium/. PAA, polyacrylamide conjugate; BT, biotin conjugate.

Glycan no. Glycan (glycotope)–spacer Glycotope abbreviation S/N
High affinity (S/N was greater than 3 times the average S/N for the entire array)
 135 GalNAcα1-3Gal–Sp2 A disaccharide 110.03
 8 α-GalNAc–Sp2 α-GalNAc 101.02
 30 Galα1-4Galβ1-4Glcβ–Sp1 Pk; (ELβ Tri-) 75.88
 4 α-D-Gal–Sp2 Galα1- 75.33
 64 Galα1-3Galβ–Sp2 B disaccharide 71.07
 193 Galα1-3Galβ1-4Glcβ–Sp1 Galili Tri; (BL Tri-) 62.93
 9 β-GalNAc–Sp2 β-GalNAc 58.25
 31 Galα1-4Galβ1-4GlcNAcβ–Sp1 P1; (EIIβ) 49.07
 134 Galα1-2Galβ–Sp2 Galα1-2Gal 46.78
 167 Galα1-3Galβ1-4(Fucα1-3)GlcNAcβ–Sp2 αGalLex; (BII/Lex Tetra-) 42.14
 138 Galα1-3Galβ1-4GlcNAcβ–Sp2 Galα1-3′LacNAc; (BIIβ Tri-) 36.69
 65 Galβ1-2Galβ–Sp2 Galβ1-2Gal 35.50
 22 GalNAcβ1-4GlcNAcβ–Sp2 Lac-di-NAc 34.51
 82 GalNAcα1-3(Fucα1-2)Galβ–Sp7 Atri-long; (Ah) 31.56
Medium affinity (S/N was greater than 2 times the average S/N for the entire array)
 172 GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAcβ–Sp2 A type II; (AhII β) 17.74
 63 6-Su-GalNAcα–Sp2 α-GalNAc-6-sulphate 16.54
 67 Galβ1-4(6-O-Su)GlcNAcβ–Sp2 6′-O-su-LacNAc 14.86
Low affinity (S/N was greater than the average S/N for the entire array)
 112 GalNAcβ1-4GlcNAcβ1-4Manα1-6(GalNAcβ1-4GlcNAcβ1- Bi-LDN (remodelled from human fibrinogen) 12.91
 4Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-N–Sp
 203 GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAc–SP1.BT A-tetra type II; (AhII Tetra-) 10.98
 26 GalNAcβ1-4(Fucα1-3)GlcNAc–Sp1 Fucα1-3-Lac-di-NAc 10.38
 91 α-D-Gal-PAA–Sp1 α-D-galactose 9.86
 77 Galα1-3(Fucα1-2)Galβ–Sp7 Btri-long; (Bh) 9.58
 93 α-GalNAc-PAA–Sp1 α-GalNAc 9.32
 202 GalNAcα1-3(Fucα1-2)Galβ1-4Glc–SP1.BT A-tetra Lac; (AhL Tetra-) 7.32