Figure 2. α-Syn binding to PC/PA liposomes modulates protein aggregation and nitration.

α-Syn–liposome complexes were incubated at 25 °C in phosphate buffer and oxidized with peroxynitrite fluxes (20 μM·min⁻¹) for 1 h; samples were taken and analysed for protein oligomer formation (A) and tyrosine nitration (B) by Western blotting using monoclonal anti-α-syn and polyclonal anti-nitrotyrosine antibodies respectively. Lane 1, native α-syn; lane 2, α-syn–PC liposomes; lane 3, α-syn–PC/PA liposomes; lane 4, α-syn, peroxynitrite; lane 5, α-syn–PC liposomes, peroxynitrite; lane 6, α-syn–PC/PA liposomes, peroxynitrite. (C) Densitometry of bands was performed for lanes 4–6, 100% being the darkest, and total intensities were plotted. *P<0.05 and ^#P<0.05 compared with the condition with α-syn–PA liposome complex.