Figure 10. Depressor response and inhibition of PI3K-C2α, Rho and MYPT1 phosphorylation induced by WMN infusion in rats.

(A) Changes in MAP induced by WMN infusion in rats. At zero time, varying doses of WMN were intravenously bolus-infused and the MAP was monitored continuously through a catheter inserted into the femoral artery. (B) Inhibition of aortic PI3K-C2α activity by WMN infusion. Rats were killed 10 min after WMN (5 mg/kg of body mass) infusion, and the aortae were removed. PI3K-C2α was immunoprecipitated and analysed for in vitro PI3K activity. (C) Inhibition of aortic Rho activity by WMN infusion. Rats were treated, and the aortae were removed in as in (B). Amounts of GTP-RhoA in the aortae were determined as in Figures 3(A)–3(C). (D) Inhibition of aortic MYPT1 Thr⁸⁵⁰ phosphorylation by WMN infusion. Rats were treated, and the aortae were removed in as in (B). Phosphorylation of MYPT1 in the aorta was determined as in Figure 3(D).