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. 2006 Feb 24;394(Pt 3):581–592. doi: 10.1042/BJ20051471

Figure 2. Inhibition of IM- and NA-induced contraction and MLC phosphorylation by PI3K inhibitors.

Figure 2

(A) Time-dependent changes of IM (5 μM)-induced contraction of VSM in the presence and absence of WMN and LY as in Figure 1(A). (B) Time-dependent changes of NA (3 μM)-induced contraction in the presence and absence of WMN and LY as in (A). (C) Dose-dependent inhibition of NA- and phorbol-ester-induced contraction by PI3K inhibitors. VSM was stimulated with NA (3 μM) for 5 min or by phorbol 12,13-dibutyrate (PDBu) (1 μM) for 60 min in the presence of various concentrations of WMN or LY. (D) Inhibition of NA (3 μM)- and PDBu (1 μM)-induced total MLC phosphorylation by WMN (1 μM) and LY (100 μM). VSM was treated as in (C). In all panels, PI3K inhibitors were added to VSM 30 min before KCl stimulation.