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. 2006 Feb 24;394(Pt 3):581–592. doi: 10.1042/BJ20051471

Figure 4. Augmentation by a PI3K inhibitor of MLC de-phosphorylation and relaxation.

Figure 4

(A) Augmentation by LY of the MLCK inhibitor ML9-induced relaxation of KCl-contracted VSM. At 5 min after KCl (60 mM) stimulation, relaxation was induced by adding ML9 (30 μM) with or without LY (100 μM). (B) Augmentation of ML9-induced MLC de-phosphorylation by LY. VSM was treated as in (A). MLC phosphorylation levels were determined at 25 min after the addition of either ML9 (KCl+ML9) or ML9 plus LY (KCl+ML9+LY). (C) Slight inhibition of the phosphatase inhibitor calyculin A-induced contraction by LY. LY (100 μM) was added 30 min before calyculin A addition. (D) No inhibition of calyculin A-induced MLC phosphorylation by LY. VSM was treated as in (C). ns, no statistical significance.