Skip to main content
. 2006 Feb 24;394(Pt 3):581–592. doi: 10.1042/BJ20051471

Figure 5. Expression of PI3K-C2α in the aortae and cultured cells, and its regulation and sensitivity to a PI3K inhibitor.

Figure 5

(A) Expression of PI3K isoforms in the aorta and cultured cells. Homogenates of rabbit and rat aortae and spleens (100 μg of protein each) and several different cultured cell types (30 μg of protein each), including rat aortic VSMCs, were analysed by immunoblotting analysis using isoform-specific antibodies. (B) Immunohistochemical detection of PI3K-C2α in VSM and endothelial cells in the rabbit aortic tissue. The aortic tissue sections were stained using the elastica van Gieson (EVG) method (top panel), antigen peptide-non-pre-absorbed (middle panel) and pre-absorbed (bottom panel) anti-PI3K-C2α antibodies. The dotted box areas in the insets were magnified. M, media; EC, endothelium. Scale bar, 20 μm.