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. 2006 Feb 24;394(Pt 3):581–592. doi: 10.1042/BJ20051471

Figure 6. The sensitivity of PI3K isoforms and Akt to a PI3K inhibitor.

Figure 6

(A) Different in vitro sensitivities of p110α, p110β and PI3K-C2α to WMN. The PI3K isoforms were immunoprecipitated from rabbit aortae, followed by in vitro PI3K assay in the presence of the indicated concentrations of WMN. (B) Lower sensitivity of PI3K-C2α to WMN in vivo in VSM. Intact VSM was treated with the indicated concentrations of WMN for 30 min, and then PI3K-C2α was immunoprecipitated from WMN-treated VSM followed by in vitro PI3K assay. Above the histograms are autoradiographs of the PI(3)P band and protein bands of PI3K-C2α in portions of immunoprecipitates. Sensitivity of (C) p110α and (D) Akt phosphorylation to WMN in vivo in VSM. In (C) and (D), VSM was pretreated with WMN as in (B). In (D), VSM was stimulated with PDGF-B chain (30 ng/ml) for 10 min at 20 min after WMN addition.*, P<0.01 compared with WMN non-treated control.