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. 2006 Feb 24;394(Pt 3):635–645. doi: 10.1042/BJ20051593

Figure 5. Trypanosome PGP is a cytosolic enzyme that is released into the plasma of infected rats.

Figure 5

(A–C) Trypanosome PGP is a cytosolic enzyme. T. brucei brucei cell bodies were fractionated into crude nuclear (N), large granule (LG), small granule (SG), crude microsomal (M) and high-speed soluble supernatant (S) fractions. The specific activities of tyrosine aminotransferase (A), α-mannosidase (B) and PGP (C), in subcellular fractions, are expressed relative to the specific activity (RSA) of the homogenate (primary y-axis). Similarly, specific activities of these three enzymes in T. brucei brucei cell-culture supernatants (SNT) are expressed relative to the specific activity (RSA) in naïve media (secondary y-axis). (D) Immunoreactive PGP is present in the plasma of infected rats. Partially fractionated plasma, from three healthy rats (lanes 1–3) or three trypanosome-infected rats (lanes 4–6), was resolved by reducing Tris/Tricine SDS/PAGE on an SDS/15% polyacrylamide gel, transfer on to a PVDF membrane and probing with anti-trypanosome PGP antibodies.