Table 1. Purification of PGP from T. brucei brucei-infected rat plasma.

The PGP activity of fractions was assayed against 10 μM Glp-AMC in 50 mM Tris/HCl/1 mM EDTA/1 mM TCEP (pH 7.4). AFU, arbitrary fluorescence units.

Fraction	Activity (ΔAFU·s−1)	Specific activity (ΔAFU·s−1·mg−1)	Purification (fold change)	Yield (%)
Whole plasma	11454	2.16	1.0	100
TPP	10538	3.25	1.5	92
Cibacron Blue F3GA–Sepharose	9007	5.35	2.4	79
HiLoad™ 26/10 Q-Sepharose	7112	65.25	30.2	62
HiPrep™ 16/10 phenyl-Sepharose	4023	1341	621	35
RESOURCE™ Q-Sepharose	3766	4570	2115	33
Sephacryl S-100 HR	3126	30057	9248	27