Table 1. Purification of GlcNase from culture of recombinant Strep. lividans pFD666-csxA.
Step | Total activity (units) | Total protein (mg) | Specific activity (units/mg) | Yield (%) | Purification (fold) |
---|---|---|---|---|---|
Crude extract | 573 | 52.8 | 10.9 | 100 | − |
SP-Sepharose | 548 | 23.9 | 22.9 | 95.6 | 2.1 |
Hydroxyapatite | 277 | 6.9 | 40.1 | 48.3 | 3.7 |