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. 2006 Feb 24;394(Pt 3):675–686. doi: 10.1042/BJ20051436

Table 1. Purification of GlcNase from culture of recombinant Strep. lividans pFD666-csxA.

Step Total activity (units) Total protein (mg) Specific activity (units/mg) Yield (%) Purification (fold)
Crude extract 573 52.8 10.9 100
SP-Sepharose 548 23.9 22.9 95.6 2.1
Hydroxyapatite 277 6.9 40.1 48.3 3.7