Table 2. Strictly conserved residues in the GH2 family.

These residues are strictly conserved according to an extensive alignment (not shown) including all the proteins from Figure 6 and Supplementary Figure 1 (http://www.BiochemJ.org/bj/394/bj3940675add.htm).

	Conserved residues
Enzyme… Species…	β-Glucuronidase (LacZ) H. sapiens	β-Galactosidase (Man2A) E. coli	β-Mannosidase (CsxA) C. fimi	β-Glucosaminidase Amy. orientalis	Remarks
	Gly347	Gly353	Gly326	Gly360	First identification
	Asn379	Asn385	Asn358	Asn389	First identification
	Arg382	Arg388	Arg361	Arg392	Catalytic nucleophile activator [51]
	Asp397	Asp403	Asp376	Asp407	First identification
	Gly400	Gly406	Gly379	Gly410	First identification
	Glu540	Glu537	Glu519	Glu541	Catalytic nucleophile [35–39]
	Gly583	Gly564	Gly608	Gly638	First identification
	Trp587	Trp568	Trp612	Trp642	Substrate binding [52]