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. 2006 Feb 24;394(Pt 3):687–692. doi: 10.1042/BJ20051563

Figure 4. Heparin-binding analysis of hydrolysed human FSAP.

Figure 4

Hydrolysed human FSAP (hydr. FSAP, −ME) eluted from the heparin column at a similar ionic strength as native human FSAP (native FSAP). In contrast, there was no heparin binding of hydrolysed FSAP when pretreated with 100 mM ME for 15 min at room temperature (hydr. FSAP, +ME). Elution was followed by analysis of UV absorbance at 280 nm. Conductivity indicates the increase in NaCl concentration. SDS/PAGE analysis of the flow-through (lane 1), wash fraction (lane 2) and elution peak (lane 3) was performed and the proteins were detected by silver staining. F11 and F12 bands interacting with heparin are indicated with an arrow and molecular mass standards are indicated in kDa.