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. 2006 Feb 24;394(Pt 3):693–698. doi: 10.1042/BJ20050576

Figure 2. Diminution of the tyrosine phosphorylation of SH3PX1 by deletion of the C-terminus did not result from losing the tyrosine phosphorylation sites.

Figure 2

(A) HA-tagged SH3PX1, ΔC1 or ΔC1-3 was co-transfected with Myc-tagged ACK2 into COS7 cells. SH3PX1 and the truncation mutants were immunoprecipitated with anti-HA and immunoblotted with anti-HA (the top panel) and anti-PY (the middle panel). The expression of ACK2 was detected by immunoblotting the cell lysates with anti-Myc (the bottom panel). IP, immunoprecipitation; IB, immunoblotting. (B) COS7 cells were transfected with HA-tagged SH3PX1 or its truncation mutants for 36 h followed by serum starvation for 12 h and subsequently stimulated with EGF (100 ng/ml) for the indicated times. SH3PX1 and the truncation mutants were immunoprecipitated with anti-HA, and immunoblotted with anti-HA (the bottom panel) and anti-PY (the top panel).