C2C12 cells were maintained in GM (day 0). To induce the muscle differentiation, cells were transferred to DM and cultured for 1–3 days. (A) TauT, MEF2 and GAPDH mRNA expression was analysed by Northern blotting. The relative TauT band intensities were assessed as the ratio to the intensity for GAPDH. Results are means±S.E.M., n=3. (B) TauT expression was examined by Western-blot analysis with anti-TauT antibody. The relative TauT band intensities were assessed as the ratio to the intensity for GAPDH. Results are means±S.E.M., n=3. *, P<0.05 compared with myoblasts (day 0); **, P<0.01 compared with myoblasts (day 0).