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. 2006 Feb 24;394(Pt 3):699–706. doi: 10.1042/BJ20051303

Figure 4. Promoter analyses of the rat TauT gene using a deletion mutant.

Figure 4

C2C12 cells were transfected with the TauT promoter-reporter plasmid (pTauT/−862-luc, pTauT/−834-luc, pTauT/−269-luc or pTauT−99-luc) and pRL-TK. After 18 h of cultivation in GM, cells were cultured in DM for 2 days. The promoter activity of the TauT gene was measured and normalized to the luciferase activity of pRL-TK. pl-luc basic vector was used as a promoterless control. Results are means±S.E.M., n=3. The putative binding sites of MEF2, the E-box for bHLH transcription factors, and SRF in the −862/+46 TauT promoter region are shown. The experiments were repeated three times with similar results. *, P<0.05; **, P<0.01.