Figure 2. Example of the calpain-2 degradome (Template A).

A-templates from naïve hippocampus (upper panel) and calpain-2-digested hippocampal lysate (lower panel) were compared in triplicate (9 comparisons in total). One set of representative blots is shown. MM markers (lane 40) are indicated on the right. Protein bands with sufficient intensity were subsequently decoded and quantified using computer software as described in the Experimental section. We noted that for a number of proteins (solid box; upper panel), their average intensity decreased more than 2-fold after calpain digestion and several BDPs were also observed (BDP, dotted box; lower panel). ZO, zona occludin; for further definitions see Table 1, legend.