Figure 6. An HTPI approach allows rapid target confirmation.

(A) Extracted lanes (E7) from template E of the HTPI gel: intact βII-spectrin (240 kDa) expression level was shown to be significantly decreased by calpain-2 digestion, caspase-3 digestion and after TBI. A calpain-mediated BDP of 110 kDa (black label) and 2 caspase-mediated BDPs of 108 and 85 kDa respectively (grey labels) were tentatively identified. These 3 BDPs were also tentatively identified in the TBI samples. (B) Traditional SDS/PAGE and Western blotting were also performed using identical monoclonal anti-βII-spectrin antibodies. Samples analysed were naïve (pooled) versus calpain-2 and capase-3 digestion (left 3 lanes), as well as 4 separate naïve and TBI samples. Again, BDPs of 110 kDa (solid arrow) and of 108 and 85 kDa (open arrow heads) were observed. * Indicates rat heavy-chain IgG and fragments from contaminating blood that cross-react with the secondary anti-(mouse IgG) antibody detection system used.