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. 1991 Nov;74(3):490–496.

Specificity of the immune response to the group B polysaccharide of Neisseria meningitidis.

M R Lifely 1, J Esdaile 1
PMCID: PMC1384645  PMID: 1722773

Abstract

A panel of monoclonal antibodies (mAb) and polyclonal sera of murine, human and equine origin, of IgM isotype and with specificity for Neisseria meningitidis group B polysaccharide, an alpha(2----8)-linked homopolymer of sialic acid, were examined for their antigenic and biological specificities. The nature of the antigenic determinants on B polysaccharide was investigated using a series of N-acyl derivatives of B polysaccharide, two sialic acid polymers containing alpha(2----9)-linkages and a series of polynucleotides. The panel of antibodies recognized an array of unrelated antigenic determinants on the B polysaccharide, despite its structural simplicity, and all but one were highly effective in an in vitro bactericidal assay and/or in an in vivo murine passive protection model. There was no evidence that B polysaccharide induced antibody capable of blocking biological activity (blocking antibody).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhattacharjee A. K., Jennings H. J., Kenny C. P., Martin A., Smith I. C. Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. J Biol Chem. 1975 Mar 10;250(5):1926–1932. [PubMed] [Google Scholar]
  2. Brandt B. L., Wyle F. A., Artenstein M. S. A radioactive antigen-binding assay for Neisseria meningitidis polysaccharide antibody. J Immunol. 1972 Apr;108(4):913–920. [PubMed] [Google Scholar]
  3. Gotschlich E. C., Liu T. Y., Artenstein M. S. Human immunity to the meningococcus. 3. Preparation and immunochemical properties of the group A, group B, and group C meningococcal polysaccharides. J Exp Med. 1969 Jun 1;129(6):1349–1365. doi: 10.1084/jem.129.6.1349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Husmann M., Roth J., Kabat E. A., Weisgerber C., Frosch M., Bitter-Suermann D. Immunohistochemical localization of polysialic acid in tissue sections: differential binding to polynucleotides and DNA of a murine IgG and a human IgM monoclonal antibody. J Histochem Cytochem. 1990 Feb;38(2):209–215. doi: 10.1177/38.2.1688896. [DOI] [PubMed] [Google Scholar]
  5. Häyrinen J., Bitter-Suermann D., Finne J. Interaction of meningococcal group B monoclonal antibody and its Fab fragment with alpha 2-8-linked sialic acid polymers: requirement of a long oligosaccharide segment for binding. Mol Immunol. 1989 Jun;26(6):523–529. doi: 10.1016/0161-5890(89)90003-5. [DOI] [PubMed] [Google Scholar]
  6. Jennings H. J., Roy R., Gamian A. Induction of meningococcal group B polysaccharide-specific IgG antibodies in mice by using an N-propionylated B polysaccharide-tetanus toxoid conjugate vaccine. J Immunol. 1986 Sep 1;137(5):1708–1713. [PubMed] [Google Scholar]
  7. Jennings H. J., Roy R., Michon F. Determinant specificities of the groups B and C polysaccharides of Neisseria meningitidis. J Immunol. 1985 Apr;134(4):2651–2657. [PubMed] [Google Scholar]
  8. Kabat E. A., Liao J., Osserman E. F., Gamian A., Michon F., Jennings H. J. The epitope associated with the binding of the capsular polysaccharide of the group B meningococcus and of Escherichia coli K1 to a human monoclonal macroglobulin, IgMNOV. J Exp Med. 1988 Aug 1;168(2):699–711. doi: 10.1084/jem.168.2.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kabat E. A., Nickerson K. G., Liao J., Grossbard L., Osserman E. F., Glickman E., Chess L., Robbins J. B., Schneerson R., Yang Y. H. A human monoclonal macroglobulin with specificity for alpha(2----8)-linked poly-N-acetyl neuraminic acid, the capsular polysaccharide of group B meningococci and Escherichia coli K1, which crossreacts with polynucleotides and with denatured DNA. J Exp Med. 1986 Aug 1;164(2):642–654. doi: 10.1084/jem.164.2.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Krambovitis E., McIllmurray M. B., Lock P. A., Holzel H., Lifely M. R., Moreno C. Murine monoclonal antibodies for detection of antigens and culture identification of Neisseria meningitidis group B and Escherichia coli K-1. J Clin Microbiol. 1987 Sep;25(9):1641–1644. doi: 10.1128/jcm.25.9.1641-1644.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lifely M. R., Gilbert A. S., Moreno C. Rate, mechanism, and immunochemical studies of lactonisation in serogroup B and C polysaccharides of Neisseria meningitidis. Carbohydr Res. 1984 Dec 1;134(2):229–243. doi: 10.1016/0008-6215(84)85040-5. [DOI] [PubMed] [Google Scholar]
  12. Lifely M. R., Gilbert A. S., Moreno C. Sialic acid polysaccharide antigens of Neisseria meningitidis and Escherichia coli: esterification between adjacent residues. Carbohydr Res. 1981 Aug 1;94(2):193–203. doi: 10.1016/s0008-6215(00)80717-x. [DOI] [PubMed] [Google Scholar]
  13. Lifely M. R., Lindon J. C., Williams J. M., Moreno C. Structural and conformational features of the Escherichia coli K92 capsular polysaccharide. Carbohydr Res. 1985 Nov 1;143:191–205. doi: 10.1016/s0008-6215(00)90708-0. [DOI] [PubMed] [Google Scholar]
  14. Lifely M. R., Roberts S. C., Shepherd W. M., Esdaile J., Wang Z., Cleverly A., Aulaqi A. A., Moreno C. Immunogenicity in adult males of a Neisseria meningitidis group B vaccine composed of polysaccharide complexed with outer membrane proteins. Vaccine. 1991 Jan;9(1):60–66. doi: 10.1016/0264-410x(91)90318-z. [DOI] [PubMed] [Google Scholar]
  15. Lifely M. R., Wang Z. Immune responses in mice to different noncovalent complexes of meningococcal B polysaccharide and outer membrane proteins. Infect Immun. 1988 Dec;56(12):3221–3227. doi: 10.1128/iai.56.12.3221-3227.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lindon J. C., Vinter J. G., Lifely M. R., Moreno C. Conformational and dynamic differences between N. meningitidis serogroup B and C polysaccharides, using n.m.r. spectroscopy and molecular mechanics calculations. Carbohydr Res. 1984 Oct 1;133(1):59–74. doi: 10.1016/0008-6215(84)85183-6. [DOI] [PubMed] [Google Scholar]
  17. Masson L., Holbein B. E. Influence of nutrient limitation and low pH on serogroup B Neisseria meningitidis capsular polysaccharide levels: correlation with virulence for mice. Infect Immun. 1985 Feb;47(2):465–471. doi: 10.1128/iai.47.2.465-471.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Michon F., Brisson J. R., Jennings H. J. Conformational differences between linear alpha (2----8)-linked homosialooligosaccharides and the epitope of the group B meningococcal polysaccharide. Biochemistry. 1987 Dec 15;26(25):8399–8405. doi: 10.1021/bi00399a055. [DOI] [PubMed] [Google Scholar]
  19. Moreno C., Hewitt J., Hastings K., Brown D. Immunological properties of monoclonal antibodies specific for meningococcal polysaccharides: the protective capacity of IgM antibodies specific for polysaccharide group B. J Gen Microbiol. 1983 Aug;129(8):2451–2456. doi: 10.1099/00221287-129-8-2451. [DOI] [PubMed] [Google Scholar]
  20. Moreno C., Lifely M. R., Esdaile J. Immunity and protection of mice against Neisseria meningitidis group B by vaccination, using polysaccharide complexed with outer membrane proteins: a comparison with purified B polysaccharide. Infect Immun. 1985 Feb;47(2):527–533. doi: 10.1128/iai.47.2.527-533.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rice P. A., Vayo H. E., Tam M. R., Blake M. S. Immunoglobulin G antibodies directed against protein III block killing of serum-resistant Neisseria gonorrhoeae by immune serum. J Exp Med. 1986 Nov 1;164(5):1735–1748. doi: 10.1084/jem.164.5.1735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rubinstein L. J., Stein K. E. Murine immune response to the Neisseria meningitidis group C capsular polysaccharide. II. Specificity. J Immunol. 1988 Dec 15;141(12):4357–4362. [PubMed] [Google Scholar]
  23. Schiffer M. S., Oliveira E., Glode M. P., McCracken G. H., Jr, Sarff L. M., Robbins J. B. A review: relation between invasiveness and the K1 capsular polysaccharide of Escherichia coli. Pediatr Res. 1976 Feb;10(2):82–87. doi: 10.1203/00006450-197602000-00002. [DOI] [PubMed] [Google Scholar]
  24. Wyle F. A., Artenstein M. S., Brandt B. L., Tramont E. C., Kasper D. L., Altieri P. L., Berman S. L., Lowenthal J. P. Immunologic response of man to group B meningococcal polysaccharide vaccines. J Infect Dis. 1972 Nov;126(5):514–521. doi: 10.1093/infdis/126.5.514. [DOI] [PubMed] [Google Scholar]
  25. Zollinger W. D., Mandrell R. E. Importance of complement source in bactericidal activity of human antibody and murine monoclonal antibody to meningococcal group B polysaccharide. Infect Immun. 1983 Apr;40(1):257–264. doi: 10.1128/iai.40.1.257-264.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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