Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1988 Jul;64(3):559–561.

Platelet-activating factor induces eosinophil peroxidase release from purified human eosinophils.

C Kroegel 1, T Yukawa 1, G Dent 1, P Chanez 1, K F Chung 1, P J Barnes 1
PMCID: PMC1385073  PMID: 3410498

Abstract

The degranulation response of purified human eosinophils to platelet-activating factor (PAF) has been studied. PAF induced release of eosinophil peroxidase (EPO) and beta-glucuronidase from highly purified human eosinophils with an EC50 of 0.9 nM. The order of release was comparable with that induced by phorbol myristate acetate (PMA). The new specific PAF antagonist 3-[4-(2-chlorophenyl)-9-methyl-H-thieno[3,2-f] [1,2,4]triazolo-[4,3a][1,4]-diazepin-2-yl](4-morpholinyl)- 1-propane-one (WEB 2086) inhibited the PAF-induced enzyme release by human eosinophils in a dose-dependent manner. The viability of eosinophils were unaffected both by PAF and WEB 2086. The results suggest that PAF may amplify allergic and inflammatory reactions by release of preformed proteins from eosinophil granules.

Full text

PDF
559

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agosti J. M., Altman L. C., Ayars G. H., Loegering D. A., Gleich G. J., Klebanoff S. J. The injurious effect of eosinophil peroxidase, hydrogen peroxide, and halides on pneumocytes in vitro. J Allergy Clin Immunol. 1987 Mar;79(3):496–504. doi: 10.1016/0091-6749(87)90368-x. [DOI] [PubMed] [Google Scholar]
  2. Carlson M. G., Peterson C. G., Venge P. Human eosinophil peroxidase: purification and characterization. J Immunol. 1985 Mar;134(3):1875–1879. [PubMed] [Google Scholar]
  3. Casals-Stenzel J., Muacevic G., Weber K. H. Pharmacological actions of WEB 2086, a new specific antagonist of platelet activating factor. J Pharmacol Exp Ther. 1987 Jun;241(3):974–981. [PubMed] [Google Scholar]
  4. Cramer R., Soranzo M. R., Dri P., Menegazzi R., Pitotti A., Zabucchi G., Patriarca P. A simple reliable assay for myeloperoxidase activity in mixed neutrophil-eosinophil cell suspensions: application to detection of myeloperoxidase deficiency. J Immunol Methods. 1984 May 11;70(1):119–125. doi: 10.1016/0022-1759(84)90396-x. [DOI] [PubMed] [Google Scholar]
  5. FISHMAN W. H. Beta-glucuronidase. Adv Enzymol Relat Subj Biochem. 1955;16:361–409. doi: 10.1002/9780470122617.ch7. [DOI] [PubMed] [Google Scholar]
  6. Fukuda T., Ackerman S. J., Reed C. E., Peters M. S., Dunnette S. L., Gleich G. J. Calcium ionophore A23187 calcium-dependent cytolytic degranulation in human eosinophils. J Immunol. 1985 Aug;135(2):1349–1356. [PubMed] [Google Scholar]
  7. Glauert A. M., Oliver R. C., Thorne K. J. The interaction of human eosinophils and neutrophils with non-phagocytosable surfaces: a model for studying cell-mediated immunity in schistosomiasis. Parasitology. 1980 Jun;80(3):525–537. doi: 10.1017/s0031182000000986. [DOI] [PubMed] [Google Scholar]
  8. Gleich G. G. The role of the eosinophilic leukocyte in bronchial asthma. Bull Eur Physiopathol Respir. 1986;22 (Suppl 7):62–69. [PubMed] [Google Scholar]
  9. Gleich G. J., Adolphson C. R. The eosinophilic leukocyte: structure and function. Adv Immunol. 1986;39:177–253. doi: 10.1016/s0065-2776(08)60351-x. [DOI] [PubMed] [Google Scholar]
  10. Henderson W. R., Chi E. Y., Klebanoff S. J. Eosinophil peroxidase-induced mast cell secretion. J Exp Med. 1980 Aug 1;152(2):265–279. doi: 10.1084/jem.152.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jong E. C., Klebanoff S. J. Eosinophil-mediated mammalian tumor cell cytotoxicity: role of the peroxidase system. J Immunol. 1980 Apr;124(4):1949–1953. [PubMed] [Google Scholar]
  12. Khalife J., Capron M., Cesbron J. Y., Tai P. C., Taelman H., Prin L., Capron A. Role of specific IgE antibodies in peroxidase (EPO) release from human eosinophils. J Immunol. 1986 Sep 1;137(5):1659–1664. [PubMed] [Google Scholar]
  13. Khalife J., Capron M., Grzych J. M., Bazin H., Capron A. Extracellular release of rat eosinophil peroxidase (EPO) I. Role of anaphylactic immunoglobulins. J Immunol. 1985 Mar;134(3):1968–1974. [PubMed] [Google Scholar]
  14. Kroegel C., König W., Mollay C., Kreil G. Generation of the eosinophil chemotactic factor (ECF) from various cell types by melittin. Mol Immunol. 1981 Mar;18(3):227–236. doi: 10.1016/0161-5890(81)90089-4. [DOI] [PubMed] [Google Scholar]
  15. Prin L., Charon J., Capron M., Gosset P., Taelman H., Tonnel A. B., Capron A. Heterogeneity of human eosinophils. II. Variability of respiratory burst activity related to cell density. Clin Exp Immunol. 1984 Sep;57(3):735–742. [PMC free article] [PubMed] [Google Scholar]
  16. Strath M., Warren D. J., Sanderson C. J. Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation factors. J Immunol Methods. 1985 Nov 7;83(2):209–215. doi: 10.1016/0022-1759(85)90242-x. [DOI] [PubMed] [Google Scholar]
  17. Vadas M. A., David J. R., Butterworth A., Pisani N. T., Siongok T. A. A new method for the purification of human eosinophils and neutrophils, and a comparison of the ability of these cells to damage schistosomula of Schistosoma mansoni. J Immunol. 1979 Apr;122(4):1228–1236. [PubMed] [Google Scholar]
  18. WROBLEWSKI F., LADUE J. S. Lactic dehydrogenase activity in blood. Proc Soc Exp Biol Med. 1955 Oct;90(1):210–213. doi: 10.3181/00379727-90-21985. [DOI] [PubMed] [Google Scholar]
  19. Wardlaw A. J., Moqbel R., Cromwell O., Kay A. B. Platelet-activating factor. A potent chemotactic and chemokinetic factor for human eosinophils. J Clin Invest. 1986 Dec;78(6):1701–1706. doi: 10.1172/JCI112765. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES