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. 1988 May;64(1):129–134.

Appearance of granule-associated molecules during activation of cytolytic T-lymphocyte precursors by defined stimuli.

J A Garcia-Sanz 1, F Velotti 1, H R MacDonald 1, D Masson 1, J Tschopp 1, M Nabholz 1
PMCID: PMC1385197  PMID: 3260214

Abstract

Lysis of target cells by cytolytic T lymphocytes (CTL) is associated with the exocytosis of cytoplasmic granules. Purified granules from CTL cell lines contain a pore-forming protein (perforin), tree serine esterases, granzyme A (60,000 MW), granzyme B (29,000 MW), and granzyme C (27,000 MW). We have compared the kinetics of appearance of cytolytic activity with that of perforin and granzyme A activity during activation of lymphocytes from normal animals with leukoagglutinin (LA) and recombinant interleukin-2 (rIL-2). Unstimulated lymph node cells do not express any of these activities, which appear between Day 3 and Day 4 of stimulation and increase rapidly to reach a pronounced peak on Day 6. On Day 7 all the activities are considerably lower, even though the cells still proliferate exponentially. There is a good correlation between the kinetics of appearance of all of these activities. Using antisera against perforin and against granzyme C, one can detect positive cytoplasmic granules in a small fraction of cells on Day 3; by Day 5, 80-90% of the cells are stained. This proportion decreases again on Day 7.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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