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ECD spectra of ubiquitin ions at 25°C. Vertical bars: black segment, c ions; open, z⋅ ions; gray filled and open, c and z⋅, respectively, from IRMPD of (M + nH)(n-1)+• ions. Vertical lines: basic residues of highest protonation probability; estimated intrinsic gas phase basicities of an amino acid in a protein: Arg, 251; His, 237; Lys, 237; and Pro, 227 kcal/mol (38). Some of these data are reprinted with permission from figure 3 of ref. 28 [Copyright (2002) American Chemical Society].
