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. 1989 Aug;67(4):531–533.

Rat mesangial cells actively produce phosphatidy linositolanchored Thy-1

T Miyata, K Isobe, R Inagi, R Taguchi, H Ikezawa, I Takai, Y Fujita, T Iwamoto, T Hasegawa, O Oda, N Yamanaka, S Sugiyama, K Maeda, K Yamada, I Nakashima
PMCID: PMC1385326  PMID: 2570037

Abstract

We provide evidence that the mesangial cells of rat kidney glomeruli express Thy-1 as a phosphatidylinositol-anchored protein. Both the mesangial area of kidney, examined in tissue sections, and mesangial cells maintained in culture for more than 3 months, showed prominent immunofluorescence staining with an anti-Thy-1 monoclonal antibody (OX7); this staining was almost completely abolished by pretreating kidney sections or mesangial cells with the phosphatidy-linositol-specific enzyme, phospholipase C. By Northern blotting, mesangial cells were shown to express mRNA of an appropriate size, hybridizing to a mouse Thy-1.1-specific probe.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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