Fig 4.

Growth at different pHs of yeast strain YPC-1 transformed with plasmids bearing the genes coding for the H⁺-PPases of A. thaliana and C. aurantiacus. YPC-1 cells were transformed with the indicated plasmids and grown in liquid medium as in Fig. 1. Dilutions (10³- and 10⁴-fold) of the cultures were made in sterile water and spotted onto agar plates made in glucose-containing rich-selective medium at the indicated pH values, adjusted as described in Materials and Methods. Growth was recorded after 5 days.