Fig 1.
Probing the oligomeric structure of SAA2.2. (A) GCL of SAA2.2 (0.1 mg/ml). The last lane (0.6*) shows cross-linking of a refolded SAA2.2 sample after it was unfolded in 6 M urea. (B) Size-exclusion HPLC of SAA2.2 at 0.1 and 0.01 mg/ml. Molecular mass standards (MW stds.) are BSA (67 kDa), ovalbumin (43 kDa), superoxide dismutase (32 kDa), horse myoglobin (19 kDa), and ribonuclease A (14 kDa). A 10-fold dilution of the SAA2.2 sample, from 0.10 to 0.01 mg/ml (10–1.0 μM), shows the same retention time, suggesting a submicromolar-to-nanomolar dissociation constant (Kd).