Fig 4.

Mutations in the photoprobe attachment region do not cause glycosylation defects. (a and b) Microsomes were prepared from each mutant strain and the wild-type control. Nondenaturing immunoprecipitation was carried out. Samples eluted from protein G agarose beads were resolved by SDS/PAGE and followed by Western blot analysis using anti-Ost1p and anti-Wbp1p, respectively. (c) Spheroplasts were prepared from each mutant strain and the wild-type control. Samples were separated on SDS/PAGE and followed by Western blot analysis using anti-CPY antibody.