Fig 5.

Ost1p block mutant 64-66AAA is incorporated into the OT complex. Microsomes were prepared from the block mutant strain and the wild-type control of Ost1p. Coimmunoprecipitation was carried out. Similar amounts of each sample that had been eluted from protein G agarose beads were analyzed by SDS/PAGE and followed by Western blot analysis using anti-Ost1p, anti-Wbp1p, anti-Swp1p, and anti-Stt3p antibodies as shown. The multiple bands of Wbp1p in lane 1 are believed to be caused by the underglycosylation of the mutant. Lane 1, 64-66AAA; lane 2, wild-type control.